Sains Malaysiana 52(10)(2023): 2829-2841
http://doi.org/10.17576/jsm-2023-5210-08
Polyunsaturated Fatty Acids from Eel
Extract Attenuate Gastritis and Hepatotoxic by Indomethacin-Induced in Rats
(Asid Lemak Politaktepu daripada Ekstrak Belut Melemahkan Gastritis dan Hepatotoksik oleh Aruhan Indometasin pada Tikus)
HERU SASONGKO1,*, AULIA
YULFA BRILIAN1, HARDIANA FEGA OCTAFIANI1, NABILA RACHMA
ALFASINA1, MUHAMMAD CAHYADI2,4 & SUTARNO3
1Department of Pharmacy, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36A
Surakarta, Jawa Tengah, Indonesia, 57126
2Department of Animal Science,
Faculty of Animal Science, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36A Surakarta, Jawa Tengah, Indonesia, 57126
3Department of Biology, Faculty of
Mathematics and Natural Science Universitas Sebelas Maret, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36A
Surakarta, Jawa Tengah, Indonesia, 57126
4Halal Research Center and Services
(HRCS), Institute for Research and Community Service, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36A Surakarta, Jawa Tengah, Indonesia, 57126
Diserahkan: 8 November 2022/Diterima: 20
September 2023
Abstract
Nonsteroidal anti-inflammatory
drugs (NSAIDs) potentially induce hepatotoxicity and ulcerative lesions in the
stomach. Several studies have been conducted on the benefits of natural
resources to overcome the problem of drug side effects. However, the use of
fishery resources, such as the eel (Anguilla
bicolor), for medicine has not been widely studied. Eels are rich in
omega-3 polyunsaturated fatty acids with antioxidant and anti-inflammatory
effects. Therefore, this study aims to determine the gastroprotective and hepatoprotective effects of polyunsaturated fatty acids extracted from eel (FaE).
The experimental models were 42 male rats divided into seven groups. The
experiment was performed for 14 days by orally administering FaE samples. On the 15th day, the rats were orally induced
with indomethacin (48 mg/kg). After 8 h of induction, they were euthanized
using ketamine and xylazine. Furthermore, blood
samples were collected from the animals to measure the levels of aspartate
aminotransferase and alanine aminotransferase. Malondialdehyde (MDA) and glutathione (GSH) levels were measured, while the histology of the
liver and stomach were examined. The results showed that the administration of FaE from a dose of 1000 mg/kg significantly attenuated the
liver and stomach damage caused by indomethacin. The influence of oxidative
stress inhibition on damage is characterized by inhibition of the increase in MDA
and a decrease in GSH. This research shows that FaE has the potential to inhibit liver and gastric damage due to the use of NSAIDs.
However, further investigation needs to be conducted on other specific
parameters of the inflammatory mechanism.
Keywords: Drug injury; fish;
omega-3; oxidative stress
Abstrak
Dadah anti-radang bukan steroid berpotensi menyebabkan kehepatotoksikan dan lesi ulseratif di dalam perut. Beberapa kajian telah dijalankan tentang kebaikan sumber alam bagi mengatasi masalah kesan sampingan dadah. Walau bagaimanapun, penggunaan sumber berasaskan ikan, seperti belut (Anguilla bicolor), untuk tujuan rawatan belum dikaji dengan meluas. Belut kaya dengan asid lemak tak tepu omega-3, yang mempunyai kesan antioksidan dan anti-radang. Oleh itu, kajian ini bertujuan untuk menentukan kesan gastropelindung dan gastropelindung asid lemak tak tepu yang diekstrak daripada belut (FaE). Model uji kaji terdiri daripada 42 ekor tikus Jantan yang dibahagikan kepada tujuh kumpulan. Uji kaji ini dilakukan selama 14 hari dengan memberikan sampel FaE secara oral. Pada hari ke-15, tikus telah diinduksi dengan indometasin (48 mg/kg) secara oral. Selepas 8 jam induksi, mereka dimatikan menggunakan ketamin dan xilazina. Kemudian, sampel darah dikumpulkan daripada haiwan tersebut untuk mengukur tahap aspartate aminotransferase dan alanine
aminotransferase. Tahap malondialdehid (MDA) dan glutation (GSH) diukur, manakala histologi hati dan perut diperiksa. Hasil kajian menunjukkan bahawa pemberian FaE daripada dos 1000 mg/kg dengan ketara melemahkan kerosakan hati dan perut yang disebabkan oleh indometasin. Pengaruh perencatan tekanan oksidatif terhadap kerosakan dicirikan oleh perencatan peningkatan MDA dan penurunan GSH. Penyelidikan ini menunjukkan bahawa FaE mempunyai potensi untuk menghalang kerosakan hati dan gastrik akibat penggunaan NSAID. Walau bagaimanapun, kajian lanjut perlu dijalankan terhadap parameter khusus yang lain tentang mekanisme keradangan.
Kata kunci: Ikan; kecederaan dadah; omega-3; tekanan oksidatif
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*Pengarang untuk surat-menyurat: heru_sasongko@staff.uns.ac.id
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